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KMID : 0603820130190030266
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Journal of Experimental & Biomedical Science
2013 Volume.19 No. 3 p.266 ~ p.269
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Direct Interaction Between Akt1 and Gcn5 and its Plausible Function on Hox Gene Expression in Mouse Embryonic Fibroblast Cells
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Oh Ji-Hoon
Lee You-ra
Kong Kyoung-Ah
Kim Myoung-Hee
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Abstract
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Hox genes encode transcription factors important for anterior?posterior body patterning at early stages of embryonic
development. However, the precise mechanisms by which signal pathways are stimulated to regulate Hox gene expression are not clear. In the previous study, protein kinase B alpha (Akt1) has been identified as a putative upstream regulator of Hox genes, and Akt1 has shown to regulate Gcn5, a prototypical histone acetyltransferase (HAT), in a negative way in mouse embryonic fibroblast (MEF) cells. Since the activity of HAT such as the CBP/p300, and PCAF (a Gcn5 homolog), was down?regulated by Akt through a phosphorylation at the Akt consensus substrate motif (RXRXXS/T), the amino acid sequence of Gcn5 protein was analyzed. Mouse Gcn5 contains an Akt consensus substrate motif as RQRSQS sequence while human Gcn5 does not have it. In order to see whether Akt1 directly binds to Gcn5, immunoprecipitation with anti?Akt1 antibody was carried out in wild?type (WT) mouse embryonic fibroblast (MEF) cells, and then western blot
analysis was performed with anti?Akt1 and anti?Gcn5 antibodies. Gcn5 protein was detected in the Akt1 immunoprecipitated
samples of MEFs. This result demonstrates that Akt1 directly binds to Gcn5, which might have contributed the down
regulation of the 5’ Hoxc gene expressions in wild type MEF cells
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KEYWORD
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Hox genes, Akt1, HAT, Gcn5
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